Coding

Part:BBa_K4497002

Designed by: Till Gundlach   Group: iGEM22_Munich   (2022-10-09)


Anti-GFP nanobody

Anti-GFP nanobody is a nanobody directed against GFP [1].


When using this part consider:

Add Signaling Sequence, there is no Start Codon, there is no Stop Codon

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 241
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 241
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]: Zhang, Z., Wang, Y., Ding, Y., & Hattori, M. (2020). Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-62606-7

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